Current rice production is highly vulnerable to insect-borne viral diseases

Rice is one of the most important food crops and feeds more than half of the world’s population [1].Arthropod-borne rice viruses have caused devastating epidemics in Asian countries and are amajor threat to food security [2]. However, little is known about the vulnerability of rice crops to viral pathogens [3,4], especially southern rice black-streaked dwarf virus (SRBSDV), rice black-streaked dwarf virus (RBSDV) and rice gall dwarf virus (RGDV), known to induce annual outbreaks by insect transmission in some localities in Asia. To address this question, we first investigated the susceptibility of 136 conventional and hybrid rice varieties widely cultivated or approved for release in China to SRBSDV, which has been circulating in Asian countries since its first report in 2008 [2]. We monitored symptom development and asymptomatic infection in seedlings after inoculation with viruliferous white-backed plant hoppers (Sogatella furcifera, Horváth) in a greenhouse. We found that most of the varieties examinedwere highly susceptible to SRBSDV and developed the characteristic disease symptoms at 45 days postinoculation (Fig. 1A and Supplementary Table S1). The results from greenhouse inoculation with viruliferous insect vectors predict widespread vulnerability of rice cultivars to SRBSDV in rice fields. To test this hypothesis, we planted seedlings of 528 varieties in 3 consecutive years under open field conditions at locations of Nanning and Guilin, Guangxi Province that have recorded a multiyear SRBSDV outbreak from plant hopper transmission. We found that ≥25% of the seedlings from 80%–93% of the examined hybrid or conventional indica

Rice is one of the most important food crops and feeds more than half of the world's population [1]. Arthropod-borne rice viruses have caused devastating epidemics in Asian countries and are a major threat to food security [2]. However, little is known about the vulnerability of rice crops to viral pathogens [3,4], especially southern rice black-streaked dwarf virus (SRBSDV), rice black-streaked dwarf virus (RBSDV) and rice gall dwarf virus (RGDV), known to induce annual outbreaks by insect transmission in some localities in Asia.
To address this question, we first investigated the susceptibility of 136 conventional and hybrid rice varieties widely cultivated or approved for release in China to SRBSDV, which has been circulating in Asian countries since its first report in 2008 [2]. We monitored symptom development and asymptomatic infection in seedlings after inoculation with viruliferous white-backed plant hoppers (Sogatella furcifera, Horváth) in a greenhouse. We found that most of the varieties examined were highly susceptible to SRBSDV and developed the characteristic disease symptoms at 45 days postinoculation (Fig. 1A and Supplementary  Table S1).
The results from greenhouse inoculation with viruliferous insect vectors predict widespread vulnerability of rice cultivars to SRBSDV in rice fields. To test this hypothesis, we planted seedlings of 528 varieties in 3 consecutive years under open field conditions at locations of Nanning and Guilin, Guangxi Province that have recorded a multiyear SRBSDV outbreak from plant hopper transmission. We found that ≥25% of the seedlings from 80%-93% of the examined hybrid or conventional indica and japonica cultivars became infected naturally with SRBSDV 60 days after seedling transplantation (Fig. 1A Fig. S1 and Supplementary Table S2). These results indicate that rice varieties currently in production and promotion generally lack broad-spectrum resistance to rice viruses.
To assess the impact of viral infection, 10 seedlings from each of 20 varieties randomly selected either uninfected or infected with RBSDV or RGDV were grown to maturity for calculating seed setting rate (SSR). We found that infection with either RBSDV or RGDV drastically decreased the SSR of both conventional and hybrid cultivars ( Fig. 1C and Supplementary Table S3). Together, our findings reveal that rice production and food security are dangerously vulnerable to potential epidemics caused by any of the three insect-borne viral pathogens.
We further used a recently developed methodology framework (3VmrMLM) of genome-wide association studies (GWAS) to search for QTNs (quantitative trait nucleotides) loci associated with viral tolerance based on the data sets from Nanning and Guilin and the available genomic sequences of rice varieties [5][6][7][8]. Seven QTNs located on chromosomes 1, 2, 4, 7 and 12 were found to be significantly associated with viral tolerance (Fig. 1D and Supplementary Table S4). Thus, it seems possible to investigate the genetic mechanisms of rice viral resistance using the experimental system established in this work as shown recently in Arabidopsis [9].
Major advances have been made in understanding the mechanisms of important breeding traits of rice such as high yield and rice blast disease resistance [10][11][12][13][14][15]. Current rice-breeding programs demand resistance evaluation against major fungal and bacterial pathogens. However, the direct link between the agro-ecosystem changes and the outbreaks of rice virus diseases needs attention. Our study shows that the rice varieties popularized in production generally lack antiviral function under both natural and greenhouse conditions and viral infection significantly reduce rice production. Based on our findings, we conclude that there is an urgency to develop a standardized protocol for assessing the performance of current and future rice varieties against key insectborne viral pathogens to be incorporated into variety certification and approval guidelines.